Pure Cultures Lab Essay

Introduction : Pure cultures are made of only one type of organisms and can be used to study their properties. A method used to isolate pure cultures is making a steak-plate, which is a dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod, culture was taken from the tube and dragged across area 1 several time,of the agar. The agar was then turned 90Â º, and the loop was flamed and cooled. Taking some culture from area 1, it was dragged over area two,and the same steps were done for areas 3 and 4.Another technique used was spread-plate, where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod was dipped in 95% ethyl alcohol and flamed to sterlize. The nutrient agar was then placed on the plate, and spread with rod. An environmental plate was used to test the cultures of a random object, in our experiment, it was the ocular lens of a microscope. A cotton swab was dipped into sterile water, and a random item of our choice was swabbed. After mixing the swab back in the water, the contaminated water was applied to a spread plate. Results: See attached Discussion: All the plates were successful is isolating the pure cultures except the environmental. The reason for this may have been that there was no bacteria, due to the fact they had been recently cleaned. The slant agars were able to pick up on the bacteria to show the growth. The vial that had bright yellow bacteria growing was M.leuteus, showing the successful isolation and identification. Other vials that had M.Letues and S.marcescenes had a very slight shade of bacteria growth. Questions: 1. No because a when a broth culture is used, it has not been inoculated from a pure culture, the only way would be to use a streaking method or spread plate. A mix culture slant is hard to isolate, because bacteria is clumped together, getting a single colony is difficult. These may cause contamination to the bacteria during the inoculation period. 2. If there was more culture in quadrant 4 than 3, it is due to the loop being dragged back into quadrant 1. The nutrient agar that was in 1 came back to 4, and showed more culture.

Infection Control Essay

This is a reflective assignment that will reflect upon a scenario that took place during the clinical placement of a student nurse in theatres. The assignment will focus on MRSA protocols in theatres, why it is important and how the use of reflection assists in the continuous professional development of a student nurse. Throughout the assignment the author will use the Rolfe reflective framework (2001, cited in Jasper, 2003) to explore what happened through out the scenario using the three stages of what, so what and now what. Reflection is a useful tool which health care professionals use to improve their skills and advance their future practice by revisiting scenarios that occurred and exploring why something happened, whether it could be improved in any way or to recognize good practice (Jasper & Rosser, 2013); In accordance to The Code (NMC, 2008) which dictates that all patient information should be kept confidential, and the Data protection Act (1998) which also governs that patient information should be kept secure and released to only those necessary or to who the patient shares it with; All patient details will be kept private and the patient is referred to only as the patient. During a surgical procedure in theatre the MRSA protocol was being followed. However during the procedure a Doctor left the theatre and then tried to re enter the preparation room, the â€Å"clean† circulating nurse did not allow this and informed the doctor to change scrubs and wash their shoes straight away. A full description of the scenario is attached as Appendix 1. The nurse was trying to minimize risk of cross infection as clothing and footwear could be contaminated by surfaces (Kumar, Saunders & Watson, 2000). The student nurse needs to have a good understanding of MRSA, protocols and research and evidence based approaches to deliver safe and appropriate care whilst minimizing risk of cross infection. Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that is resistant to a number of antibiotics that treat infection including methicillin. There are different strains of Staphylococcus aureus making it harder or easier to treat. MRSA can live in the nostrils or on skin and is  easily transferred from person to person or surface by contact, it can also be dispersed by skin particles through the air (McNeil, Mody & Bradley, 2002), contaminating beds and surfaces (Kumar, Saunders & Watson, 2000 ) and can remain active for longer than 6 months (Wagenvoort, Sluijsmans & Penders, 2000).This is why hand hygiene and environmental cleanliness is so important (Crowcroft, 1996). The bacterium can enter the bloodstream through small cuts or a wound; Depending on individuals’ immune system people can fight off lower strains of MRSA with minimal infection symptoms such as pain, temperature, dizziness etc. However people with lowered immune systems i.e. people in hospital w ith existing health complaints or infections can be harder to treat (Dougherty &Lister, 2004). MRSA is a virulent pathogen and when it doesn’t respond to treatment can cause sepsis, urinary tract infections, endocarditis, pneumonia, septic arthritis, osteomyelitis and can even result in death (Weigeit, 2007). During theatre procedures the same source isolation nursing that is used on the wards is used in theatres nursing that is used on the wards (NHS, 2012). St George’s Hospital (NHS St Georges Trust, 2012) theatre policy outlines during theatre and recovery staff numbers should be kept to a minimum, all unnecessary equipment should be removed from theatre, staff should wear protective clothing and a plastic gown, after the procedure all equipment and surfaces should be cleaned and patients should be nursed using source isolation practices. (Dougherty & Lister, 2004) Source isolation aims to keep the source of infection from spreading anywhere by means of hand washing, washing, protective clothing and incineration of all disposable waste in a controlled manor. The National Institute for clinical excellence also highlighted the importance of source isolation regarding MRSA discussing its negative impacts on health ranging from discomfort to serious disability or death. (NICE, 2011) in 2009/10 the NHS dealt with almost 1900 bloodstream infections, each infection cost the NHS around  £7000. The aim in following guidelines is to protect patients, protect staff and to protect the environment for the next patient who will enter the theatre having an operation (Dougherty & Lister, 2004). Thanks to evidence based clinical practice and improved hand hygiene MRSA bloodstream infection rates have dropped by 41% in the NHS across England  since 2009 and a further 29% in 2012/2013 (Department of Health, 2012) From this event the student was able to reflect on research and evidence based approaches specifically in regards to MRSA and gain a more in-depth understanding of MRSA. Reading about bacterium and paths of transmission helped the student nurse have a better understanding of infection control measures and their importance.

Politics and Administration Essay

Introduction 186, 207, 221? Power Struggle between the pro-democratic and pro-conservatism One of the reasons that led to such a tragic end for Tian’anmen Movement is the power struggle between the two camps of leaders, pro-democratic (Former General Secretary of the Chinese Communist Party, Zhao Ziyang) and pro-conservatism (Deng Xiao Ping). In 2009, a memoir was published based on audiotapes recorded by Zhao Ziyang, called Prisoner of the State: The Secret Journal of Premier Zhao Ziyang, he mentioned that he â€Å"didn’t want to be a General Secretary who opens fire on the people.† From this, we could clearly tell by that time, Zhao differed in opinion from Deng and other conservative leaders, like Li Peng on how to handle the student movement, i.e. whether to pursue a peaceful or a military solution. http://blog.foolsmountain.com/2009/06/03/tiananmen-1989-a-need-for-dialogue-20-years-later/ One very crucial turning point that we know is that by that time, the CCP General Secretary made a scheduled official visit to North Korea, which turned out to be a bad decision at the time of turmoil; this was because on April 26th CCP Central issued an editorial on â€Å"People’s Daily† in the absence of Zhao Ziyang, which was titled â€Å"Uphold the flag to unambiguously oppose any turmoil†, denouncing that the patriotic student movement as a turmoil ignited by an â€Å"extremely small handful of opportunists†. http://www.alliance.org.hk/64/6420/?page_id=521 According to Wu Jiaxiang, a former aide and a leading political scientist in Beijing, Zhao Ziyang, before leaving for North Korea on April 21st, had instructed that â€Å"no politburo meeting should be held in his absence† but other CCP leaders, likle Yang Shankun & Li Peng immediately convened a â€Å"CCP Expanded Poliburo Meeting†, then reported to Deng Xiaoping with meeting opinions, and then borrowed Deng Xiaoping’s mouth in stating that CCP â€Å"should not fear bloodshed†. And in Zhao Zhiyang’s self account, recorded by Yang Jisheng, he claimed that it was li Ximing & Chen Xitong who contacted Wan Li on the night of April 21st in request of convening a commissar meeting; that Wan Li relayed the info to Li Peng. Later, according to ________, Li Peng convened the meeting on the night of 24th, reported it to Deng Xiaoping on 25th, and was authorized to relay Deng’s opinions to communist cadres; and that Li Peng took advantage of the occasion to draft a newspaper commentary for publication on April 26th. By the time Zhao returned to China after the scheduled visit, he found it was just too late to remedy the situation as hostilities between the government and the people have been greatly aggravated. Other than the mis-communication over the People’s Daily’s editorial, we may gain further insights into the power struggle between the party leaders by taking a look at a previous document obtained by CNN and written by Bao Tong, a close aide to Zhao Ziyang, describing in detail the events leading up to the crackdown on student protests in Tiananmen Square on June 4, 1989. September 25, 1989 Comrade Hu Yaobang died on April 15, soon after which the student demonstrations began. I was extremely worried. Comrade Xiaoping pointed out in his speech on April 25 that the overwhelming priority in China is stability – I completely agreed with his point; I also wanted to maintain stability and prevent turmoil. Specifically, I wanted to make an effort to reduce tensions and to avoid precipitating the tension into clashes. The People’s Daily April 26th editorial, in my view, was harsh in language and lacked analysis and persuasiveness; I had my reservations about it. On May 19 and 20, the Central Committee announced the decision to send the military into Beijing and declare martial law; in my heart, I believed we made a terribly wrong move; I was afraid that we would be trapped in a very difficult situation, â€Å"riding a tiger, hard to get-off.† The Central Standing Committee collectively criticized Comrade Zhao Ziyang; I felt it was unfair. 1.1 – It was I who first informed Comrade Ziyang that the April 26th editorial had aggravated the confrontational mood of students and people who had previously taken a neutral position. Comrade Ziyang returned to Beijing from North Korea on April 30th. As soon as he returned, I reported to him as follows: Students have been demonstrating in the streets since April 27th. There were so many of them that it was impossible to blockade; there were also lots of onlookers following them. Some government officials sympathized with the demonstrations. There were signs that the incident was escalating and expanding; there were a great number of students and others who resented the April 26th editorial and believed that it was hostile towards them. I spoke about my opinion of the editorial: the positive side of the editorial was that it presented Comrade Xiaoping’s thoughts that Chin a must maintain stability and must not fall into turmoil. However, the editorial was written in a very harsh tone, did not adequately present reasons and lacked analysis. It also did not take into consideration the acceptance of people who were previously neutral. Since there were so many people who believed that the editorial was hostile to them, it was obvious that the editorial did not express its ideas clearly. Comrade Ziyang did not express his own opinions at that time. After a few days, he said to me: â€Å"It seems that there are flaws in the editorial.† Comrade Ziyang certainly formulated his opinions in his own way, but it was I who first reported to him about the editorial with this point of view. http://edition.cnn.com/2001/WORLD/asiapcf/east/04/22/tiananmen.document.01/ Students’ Uncollective Actions As discussed above, power struggle among the party leaders is one of the factors in leading to the unwanted tragedy of the June 4th Movement, but as I argue down below, the chaotic and unsystematic internal management of various student movements are by nature underlying factors to the tragedy. After the April 27 demonstration, the government commenced to open up dialogues with students, and the government seemed to take the initiative to adopt a more positive approach towards the student movement. The government’s willingness to concede and negotiate have lit up the hope for the majority of students that the entire student movement might come to a peaceful end(Source: Book) Yet, almost by the same time, the leadership and organization of the movement among the student leaders became â€Å"problematic†. Not only did the student movement organizations hold different points of views towards the movement strategies, some of the student leaders also paid no respect to organizational claims, and much often instead, they themselves acted on behalf of their organizations. One fine example would be the case of Zhou Yongjun, who was the president of Beijing Autonomous Federation. He helped organize another big demonstration on May 4. However, without any consent from his fellows and colleagues, he announced the end of class strike â€Å"without a clear resolution within the leadership†. http://www.standoffattiananmen.com/2009/04/people-of-1989-zhou-yongjun.html In order to better describe the dividing powers among the student leaders during the late stages of June 4th Movement, Zhao (2001) simply grouped the student demonstrators by that time into three groups, which are called respectively Dialogue Delegation Group, Beijing Students’ Autonomous Group and the so-called â€Å"charismatic group† http://site.ebrary.com.eproxy1.lib.hku.hk/lib/hkulibrary/docDetail.action?docID=10402626 As Zhao (2001) noted, the students that belonged to Dialogue Delegation Group were â€Å"eager for the coming dialogue with the government†. References Gonzales, Eduardo, L. and Gillespie, C.G. (1994). Presidentialism and Democratic Stability in Uruguay. In J. J. Linz and A. Valenzuela (Ed.), The Failure of Presidential Democracy, Comparative Perspective (pp. 151 – 178). Baltimore: The John Hopkins University Press Hanan, D. (2007). Presidentialism, parliamentarism and semi-presidentialism: Incentives and disincentives in achieving multiple democratic goals. Retrieved from http://djayadihanan.blogspot.com/2007/12/presidentialism-parliamentarism-and.html Linz, Juan J. (1994). The Failure of Presidential Democracy. The Case of Latin America. In J. J. Linz and A. Valenzuela (Ed.), Presidential or Parliamentary Democracy: Does it Make a Difference? (pp.7).Baltimore: The Johns Hopkins University Press Maeda, K. & Nishikawa M. (2006). Duration of Party Control in Parliamentary and Presidential Governments: A Study of Sixty-Five Democracies, 1950-1998. Retrieved from http://www.bsu.edu/web/mnishikawa/MaedaNishikawa2006CPS.pdf Mainwaring, S. (1993). Presidentialism, Multipartiism, and Democracy, The Difficult Combination. Comparative Political Studies, Vol. 26 No. 2 (pp. 198 – 228) Mainwaring, S. & Shugart, M. (1993). Juan Linz, presidentialism, and democracy: A critical appraisal. Retrieved from http://www.nd.edu/~kellogg/publications/workingpapers/WPS/200.pdf Power, Timothy and Mark J. Gasiorowski. (1997). Institutional Design and Democratic Consolidation in the Third World. Comparative Political Studies. Shugart, M. S., & Carey, J. M. (1992). Presidents and assemblies: constitutional design and electoral dynamics. Cambridge: Cambridge University Press. Valenzuela, A. (1994). Party Politics and the Crisis of Presidentialism in Chile: A Proposal for a Parliamentary Form of Government. In J. J. Linz and A. Valenzuela (Ed.), The Failure of Presidential Democracy – The Case of Latin America (pp. 93). Baltimore: The Johns Hopkins University Press

Changes in 1750 to 1850

This period of time (1750-1850) was the Industrial Revolution. This meant that the factory system and use of machines increased. There were also more wealth for Britain and increase of population! However there changes in social, religion, economic and more political changes, this resulted in incidences such as calls for education, voting, equality and so on. But the main changes were transport, industry, home/leisure and health. Transport changes quickly 1750-1850, it was an increase for much better methods of moving heavy goods such as fruit and vegetables! However the changes did not just happened at once, it had several stages. First roads were improved, than canals were built and finally the Railway was developed! This has a good impact because in 1750 travel was slow, usually on foot, but many main roads had been improved by becoming turnpikes (toll roads). Toll roads is a public or private roadway for which a fee, or toll is assessed for passage. It is a form of road pricing typically implemented to help recuperate the cost of road construction and maintenance, which on public roads amounts to a form of taxation. Journey times: London to Manchester took 4 days, London to oxford took 2 days. Over time the roads become flatter, they were not dirt tracks anymore, they made roads straighter, levelled out and they also built bridges on the roads. Canals were built so factories could move raw materials from factory to factory. The cannels had several advantages! Firstly a boat is not going to have a bumpy journey; so fragile goods are much less likely to smash on route. Secondly a canal barge is much larger than a horse drawn wagon and so it can be used to carry much more than wagons on Turnpike roads could be expected to. Also canals were cheap to use and could carry 50 tonnes! Railways developed quickly because of Stephenson! Stephenson invented steam trains. The steam trains would be used to power motors and would be used in mines to help bring coals to the surface quicker! In 1850 railways connected every major town and city in Britain, making much faster travel available for all. Journey times: London to Manchester 6 hours; London to oxford 2 hours! The most important change for the industry was the invitation of machines to do the work of hand tools. Iron was very important because it was used for machines, tools, ships, railways and trains. However the factories were making clothing and goods on time because the machinery made them quicker! The industry was now becoming more advanced. Power had changed from water to coal. Coal is very important because it power domestic homes, machinery and transport

Preparing to go global Essay Example | Topics and Well Written Essays - 750 words

Preparing to go global - Essay Example As we are planning to go international, we must know the food taste of the people of foreign countries. In the foreign countries, we should prepare the food according to the taste of the people of those countries instead of preparing it with the same ratios of salt and pepper that our people like. Today, people demand excellence and quality in whatever they buy. The companies, which provide quality products to the customers, gain success in the international markets. Understanding of the global management concept also plays a key role in the success of the companies. Global management is a technique that companies use to run the business processes efficiently in the international marketplace. While running a business worldwide, different types of cultural, political, and economic issues appear which companies need to tackle effectively in order to gain success in the international markets. We can take the example of McDonalds, a fast food chain, which has achieved success in different parts of the world. The reason behind its success is extremely talented and skilled management staff, which has a complete understanding of the global management concept. The managers of McDonalds have developed effective marketing and market-entry strategies to establish their brand all over th e world. To gain success in internationally, we would need to understand all cross-cultural issues and international business practices in order to make a permanent place in the international markets. Our management staff needs to be skilled enough to set the dimensions of success for the company. We would need to know the way to optimize profitability and promote efficiency in the global marketplace. Development of an appropriate strategic marketing plan would also play a vital role in the success of Kestrel Growth Brands. The strategic marketing plan would encompass two motives. First motive would be to attract the customers towards the

Managing People, Finance and Marketing Essay Example | Topics and Well Written Essays - 2000 words

Managing People, Finance and Marketing - Essay Example There may be organization-wide changes such as mergers and acquisitions, restructurings, leadership changes, and changes in technology. There may be the upsizing, downsizing and resizing that force organizations to change, and in some cases almost daily (Joyce, 2005). There are also changes needed in the culture of organizations or the way they do things. History is replete with many organizations changing for the better and organizations that did not and were doomed to failure in some parts of their history (Waldera, 2002). American Express. American Express, for example, had announced organization and management changes in mid- 2005. The reasons given were that changes were driven by several key developments including: the rapid growth of their Global Network Services (GNS) business in the United States and around the world; the expansion of their worldwide merchant network; the broader long-term relationships they are developing to build business with their merchant partners; and the recent entry of the Travelers Cheque business into the prepaid card arena ("American Express," 2005). Their company, they said, is in an excellent position. They would like to further strengthen their long-term position and propel the new American Express into the ranks of the most successful and most admired companies in the world ("American Express," 2005). IBM Integrated Supply Chain. ... IBM is not unique; its supply chain considerations impact upon many organizations as they attempt to find integrated solutions to complex problems. However, within IBM, this transition, which has affected organizational structure and alignment, process, and IT support, has not been without its problems. The drive to shift from a Functional to a Process control alignment has required a shift in the mindset of the organizations employees (McLaughlin, Paton & Macbeth, 2006). The IBM, as an organization needed to be able to develop flexible end-to-end (E2E) processes that can be "tweaked" and modified to meet changes in customer demand, product availability and overall performance. Performance to them is not simply down to the implementation of elaborate IT systems, but requires the alignment of key personnel in an understanding of the knowledge management aspects relating to the E2E processes. This required management to think about how the business operates from a process, as opposed to a function, perspective (McLaughlin, Paton & Macbeth, 2006). Hewlett Packard. Adizes (1988) postulated that as companies go through various life-cycle stages, their cultures need to change in order to adapt to different business challenges. Rigid adherence to a set of cultural norms can foretell disaster, especially in rapidly changing market conditions (Waldera, 2002) Consider the fate of a non-renewing organization as defined by Waldera (2002). The "HP Way" guided the success and development of this premier technology company since its inception in 1939. However effective the "HP Way" culture was in guiding the company's growth during the first fifty years of its history, it began

Unemployment and Labor Force Essay Example | Topics and Well Written Essays - 1000 words

Unemployment and Labor Force - Essay Example According to the economist Edmond Malinvaud, the type of unemployment that prevails at a particular time depends on the situation at the goods market. If the goods market is a buyers’ market in which sales are restricted by demand then Keynesian type of unemployment prevails, while if a production capacity is limited classical unemployment prevails. Common types of unemployment are as follows:1.  Frictional Unemployment: This type of unemployment occurs when a person switches from one job to another. While the person looks for another job he experiences frictional unemployment. This unemployment also applies to fresh graduates who look for jobs. It is a productive part of the economy as it increases worker’s economic efficiency and his long term welfare. This type of unemployment usually occurs due to incorrect information in the labor market due to which workers do not know what type of job they are getting hired for and hence they look for getting a new job. 2.  C lassical Unemployment: This type of unemployment occurs when real wages for available jobs are set above the market clearing level. This usually happens due to government intervention when government sets a minimum wage for a job. Even taboos especially taboos can sometimes cause wages to be set above the market clearing level (America’s Great Depression p45).3.  Structural Unemployment: This type of unemployment occurs due to mismatch between employment offered by employers and those seeking jobs.... Common types of unemployment are as follows: 1. Frictional Unemployment: This type of unemployment occurs when a person switches from one job to another. While the person looks for another job he experiences frictional unemployment. This unemployment also applies to fresh graduates who look for jobs. It is a productive part of the economy as it increases worker's economic efficiency and his long term welfare. This type of unemployment usually occurs due to incorrect information in the labor market due to which workers do not know what type of job they are getting hired for and hence they look for getting a new job. 2. Classical Unemployment: This type of unemployment occurs when real wages for available jobs are set above the market clearing level. This usually happens due to government intervention when government sets a minimum wage for a job. Even taboos especially taboos can sometimes cause wages to be set above the market clearing level (America's Great Depression p45). 3. Structural Unemployment: This type of unemployment occurs due to mismatch between employment offered by employers and those seeking jobs. It may occur due to geographical location or skill of workers or because of many other reasons. If structural unemployment occurs, frictional unemployment becomes significant as well. 4. Keynesian Unemployment: This type of unemployment occurs when there is insufficient demand in the economy. It occurs when there is a business cycle recession in the economy and wages of jobs do not fall to meet the equilibrium rate. An economy with high unemployment rate is an economy which is not using its entire available labor source which reduces its efficiency. If all the frictionally unemployed in this economy accept the first available job to them, then they